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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 25,
Number 4, May/June 2001, pp. 275-279
Here is where the title stuff goes
Simultaneous
Determination of Chlorpyrifos, Permethrin, and Their Metabolites in Rat Plasma
and Urine by High-Performance Liquid Chromatography
Aqel W. Abu-Qare and Mohamed B. Abou-Donia*
Department of Pharmacology and Cancer Biology, Duke University Medical Center,
Durham, North Carolina 27710
A high-performance
liquid chromatographic (HPLC) method was developed for the separation and quantitation
of the insecticide chlorpyrifos (O,O-diethyl-O[3,5,6-trichloro-2-pyridinyl]
phosphorothioate), its metabolites chlorpyrifos-oxon (O,O-diethyl-O[3,5,6-trichloro-2-pyridinyl]
phosphate) and TCP (3,5,6-trichloro-2-pyridinol), the insecticide permethrin
(3-(2,2-dichloro-ethenyl)-2,2-dimethylcyclopropanecarboxylic acid-(3-phenoxyphenyl)
methylester), and two of its metabolites, m-phenoxybenzyl alcohol and m-phenoxybenzoic
acid, in rat plasma and urine. The method is based on using C18 Sep-Pak®
cartridges for solid-phase extraction and reversed-phase HPLC. The compounds
were separated using a gradient of 1 to 80% acetonitrile in water (pH 3.2) at
a flow rate ranging between 1 and 1.5 mL/min in a period of 17 min and gradient
UV detection ranging between 210 and 280 nm. The retention times ranged from
9.3 to 14.5 min. The limits of detection ranged between 20 and 150 ng/mL, whereas
the limits of quantitation were 150–200 ng/mL. The respective average percentage
recoveries of chlorpyrifos, chlorpyrifos-oxon, TCP, permethrin, m-phenoxybenzyl
alcohol, and m-phenoxybenzoic were 78.6 ± 6.4, 72.8 ± 6.8, 84.8
± 8.0, 79.2 ± 8.4, 80.5 ± 7.2, and 82.3 ± 7.1 from
five spiked plasma samples and 77.5 ± 8.1, 72.8 ± 8.3, 79.9 ±
6.4, 79.1 ± 8.9, 80.5 ± 7.6, and 81.4 ± 7.8 from urine
samples. The relationship between peak areas and concentration was linear for
concentrations between 200 and 2000 ng/mL. This method was used to analyze these
chemicals and metabolites following dermal administration in rats.
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