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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 25,
Number 2, March 2001, pp. 77-80
Cobas Mira™ S Endpoint Enzymatic Assay for Plasma Formate*
B. Blomme[1]
, P. Lheureux[2], E. Gerlo[1], and V. Maes[1]
[1]Department of Clinical Chemistry–Toxicology, Academic Hospital Free
University Brussels, VUB, Laarbeeklaan 101, B-1090 Brussels, Belgium and
[2]Emergency Department, Erasme Hospital, ULB, Route de Lennik 808, B-1070 Brussels,
Belgium
In
methanol intoxication, increased levels of the metabolite formate are associated
with metabolic acidosis and an increased risk for ocular and neurological dysfunction.
A simple method for plasma formate measurement by adaptation of a manual enzymatic
assay to a Cobas Mira S analyzer is presented. Six microliters of sample is
incubated for 5 min with buffer containing nicotinamide-adenine dinucleotide.
Fifteen microliters of a suspension of formate dehydrogenase is then added.
Absorbance at 340 nm is measured every 25 s. The NADH produced when formate
is oxidized is stoichiometric to the amount of formate. The method is sensitive,
reproducible, and specific and has a broad measurement range. The frozen reagents
are stable for at least six months, so the described method can be applied to
irregular and semi-urgent requests. A recent case is reported. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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