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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 25,
Number 2, March 2001, pp. 125-129
General Unknown Screening
in Postmortem Tissue and Blood Samples: A Semi-Automatic Solid-Phase Extraction
using Polystyrene Resins Followed by Liquid–Liquid Extraction
T. Stimpfl[1],
J. Jurenitsch[2], and W. Vycudilik[1]
[1]Institute of Forensic Medicine, University of Vienna, Sensengasse 2, A-1090
Vienna, Austria and
[2]Institute of Pharmacognosy, University of Vienna, Althansstr. 14, A-1090
Vienna, Austria
The
identification of general unknown poisons in complex biological materials like
postmortem blood and tissue is a great challenge for the forensic toxicologist.
Therefore, a screening procedure utilizing a semi-automatic work-up with an
ASPEC system was developed. A broad range of different compounds can be isolated
by using non-selective and generally applicable organic polymeric sorbents such
as OASIS HLB or Isolute 101. Because colloidal solutions were applied to these
sorbents, the denaturation of proteins, which can result in an irreversible
loss of significant compounds by adsorption and occlusion, could be avoided.
Because of the process of micellar chromatography followed by liquid–liquid
extraction of the crude extract, very clean fractions were obtained from such
complex matrices as postmortem blood, liver, and brain samples. High recoveries
(72–100%) and good day-to-day relative standard deviations (1–17%)
could be achieved with both polymeric sorbents. The procedure paves the way
for the identification of general unknown poisons in target organs and is therefore
a useful tool in the field of forensic toxicology. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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