Bioanalysis of the Enantiomers of (±)-Sarin using Automated Thermal Cold-Trap Injection Combined with Two-Dimensional Gas Chromatography

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Published: Journal of Analytical Toxicology, ISSN 0146-4760, Volume 25, Number 1, January/February, pp. 57-61

Bioanalysis of the Enantiomers of (±)-Sarin using Automated Thermal Cold-Trap Injection Combined with Two-Dimensional Gas Chromatography

Helma E.T. Spruit, Hendrik C. Trap, Jan P. Langenberg*, and Hendrik P. Benschop
TNO Prins Maurits Laboratory, P.O. Box 45, 2280 AA Rijswijk, The Netherlands

A fully automated multidimensional gas chromatographic system with thermal desorption injection and alkali flame detection was developed for analysis of the enantiomers of the nerve agent (±)-sarin. The chiral stationary phase was CP Cyclodex B on which the sarin enantiomers were completely resolved. The absolute detection limit was 2.5 pg per enantiomer. The method is intended to be used for the analysis of the sarin enantiomers in biological samples. For this purpose, sarin was isolated from guinea pig blood via solid-phase extraction. Deuterated sarin was used as internal standard. Stabilization of sarin in the blood sample by acidification and addition of an excess of a competitive organophosphorus compound (neopentyl sarin) appeared to be essential. The absolute recovery of the extraction procedure was 60%, whereas the recovery relative to the internal standard was 100%.

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