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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 25,
Number 1, January/February,
pp. 35-39
Simultaneous
Determination of 2,4-D and MCPA in Canine Plasma and Urine by HPLC with Fluorescence
Detection Using 9-Anthryldiazomethane (ADAM)
Laurene
M. Dickow, Diane F. Gerken*, Richard A. Sams, and Susan M. Ashcraft
The Ohio State University, College of Veterinary Medicine, 1925 Coffey Road,
Columbus, Ohio 43210
A method
for the simultaneous determination of 2,4-dichlorophenoxyacetic acid (2,4-D)
and 2-methyl-4-chlorophenoxyacetic acid (MCPA) in canine plasma and urine has
been developed. This method used derivatization of extracted samples with 9-anthrylmethane
(ADAM) for analysis by reversed-phase high-performance liquid chromatography
with fluorescence detection. Precision and accuracy were within the accepted
limits of 15% and 85–115%, respectively, for both analytes in plasma and
urine. Calibration curves for 2,4-D and MCPA in plasma were linear (r2 >
0.99) between 0.50 and 5.0 mg/L and 5.0 and 100 mg/L. Calibration curves for
2,4-D and MCPA in urine were linear (r2 > 0.99) between 5.0 and 70.0 mg 2,4-D/L
and 10.0 and 70.0 mg MCPA/L. The lower limit of detection was 62.5 ng/mL for
both 2,4-D and MCPA.
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