Determination of Azide in Blood and Urine by Gas Chromatography

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Published: Journal of Analytical Toxicology, ISSN 0146-4760, Volume 24, Number 6, September 2000, pp. 429-432

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Determination of Azide in Blood and Urine by Gas Chromatography–Mass Spectrometry
Shigetoshi Kage1[], Keiko Kudo[2], and Noriaki Ikeda[2]
[1]Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, 7-7, Higashikoen, Hakata-ku, Fukuoka 812-8576, Japan and
[2]Department of Forensic Pathology and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan

A sensitive and simple method for determining azide in blood and urine using an extractive alkylation technique was devised. This inorganic anion was alkylated with pentafluorobenzyl bromide using tetradecyldimethylbenzylammonium chloride as the phase-transfer catalyst. 1,3,5-Tribromobenzene was used as an internal standard. The obtained derivative was analyzed by gas chromatography–mass spectrometry using the negative ion chemical ionization mode with isobutane as the reagent gas. The calibration curves for azide were linear over the concentration range from 1 to 200 nmol/mL in blood and urine, and the lower limit of detection was 0.5 nmol/mL for blood and urine. The accuracy and precision of the method were evaluated, and the coefficients of variation were found to be lower than 10%.

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