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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 24,
Number 6, September 2000, pp. 395-402
Here is where the title stuff goes
Simultaneous
Analysis of D9-THC and its Major Metabolites in Urine,
Plasma, and Meconium by GC–MS Using an Immunoaffinity Extraction Procedure
Shixia Feng1, Mahmoud A. ElSohly[1], Salvatore
Salamone[2], and Maissa Y. Salem[3]
[1]ElSohly Laboratories, Incorporated, 5 Industrial Park Drive, Oxford, Mississippi
38655
[2]Roche Diagnostics Corporation, 9115 Hague Road, Indianapolis, Indiana 46250;
and
[3]Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo,
11562, Egypt
A simple extraction procedure for D9-tetrahydrocannabinol
(D9-THC) and its metabolites from various biological specimens was developed
based on immunoaffinity chromatography. Using the affinity resin prepared by
immobilization of THC antibody onto cyanogen bromide-activated Sepharose 4B,
D9-THC and its major metabolites including 11-nor-D9-THC-9-carboxylic
acid (D9-THCCOOH), 11-hydroxy-Æ9-THC (11-OH-D9-THC),
and 8b,11-dihydroxy-D9-THC (8b,11-diOH-D9-THC)
were extracted simultaneously from plasma or urine after enzyme hydrolysis.
The samples were derivatized as TMS derivatives and analyzed by gas chromatography–mass
spectrometry in EI mode with SIM monitoring. Greater than 87% extraction recovery
of the four analytes was obtained from both plasma and urine at 5 and 50 ng/mL
concentration levels. The method was also used for meconium analysis with some
modification. The extraction recovery from meconium, however, was lower than
that of plasma and urine, ranging from 52 to 72% at the 10-ng/g level. All compounds
showed good linearity within the tested ranges up to 100 ng/mL (g). The limits
of detection ranged from 0.5 to 2.5 ng/mL in plasma and urine, and from 1.0
to 2.5 ng/g in meconium. Analysis of 24 meconium specimens showed that 11-OH-D9-THC
is indeed an important metabolite in meconium. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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