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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 24,
Number 8, November/December,
pp.696-703
Extraction and Cleanup
Methods for Analysis of Phenolic and Neutral Organohalogens in Plasma
L.
Hovander1, M. Athanasiadou1,*, L. Asplund2, S. Jensen1, and E. Klasson Wehler1,
1Department of Environmental Chemistry, Wallenberg Laboratory, Stockholm University,
SE-106 91 Stockholm, Sweden and
2Institute of Applied Environmental Research, Stockholm University, SE-106 91
Stockholm, Sweden
A
method for the analysis of potential endocrine-disrupting compounds, such as
phenolic halogenated compounds (e.g., chlorinated and brominated phenols) and
hydroxylated PCBs, in blood plasma is presented. Neutral halogenated compounds,
specifically brominated diphenyl ethers and PCBs, are also included in the evaluation.
An efficient denaturation and extraction step is described, and three methods
for lipid removal are evaluated. The latter includes a nondestructive method
based on high-resolution gel permeation chromatography (HR-GPC), a newly developed
silica gel/sulfuric acid column, and lipid removal by sulfuric acid treatment.
Recoveries, based on gas chromatography with an electron capture detector (GC–ECD),
were between 70 and 90% for most of the studied compounds. The recoveries of
phenolic compounds were generally slightly lower than those of the neutral compounds.
The sulfuric acid treatment and silica gel/sulfuric acid column gave the highest
yields for acid stable compounds, although a few target compounds were lost
during that treatment and all compounds were recovered with the HR-GPC method. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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