An Automated and Simultaneous Solid-Phase Extraction of Æ9-Tetrahydrocannabinol and 11-Nor-9-Carboxy-Æ9-Tetrahydrocannabinol from Whole Blood Using the Zymark RapidTraceª with Confirmation and Quantitation by GCÐEI-MS

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Published: Journal of Analytical Toxicology, ISSN 0146-4760, Volume 24, Number 4, May/June 2000, pp. 289-295

An Automated and Simultaneous Solid-Phase Extraction of D⁹-Tetrahydrocannabinol and 11-Nor-9-Carboxy-D⁹-Tetrahydrocannabinol from Whole Blood Using the Zymark RapidTrace™ with Confirmation and Quantitation by GC–EI-MS A sensitive, reliable, and automated solid-phase extraction (SPE) method was developed for the simultaneous extraction, confirmation, and quantitation of Æ9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-Æ9-tetrahydrocannabinol (THCCOOH) from whole blood. The extraction was performed on the Zymark RapidTrace SPE Workstation with a reduced solvent volume SPE copolymer cartridge. Quantitative analysis was completed on a benchtop gas chromatograph–mass spectrometer using electron ionization mode with selected ion monitoring of three ions for each analyte. The limits of quantitation for THC and THCCOOH were 2 ng/mL and 1 ng/mL, respectively. The limits of detection for THC and THCCOOH were 1.6 ng/mL and 0.8 ng/mL, respectively. Extensive method validation is presented including extraction recoveries, within-run precision, day-to-day precision, linearity, and carryover. This procedure is used routinely in the laboratory on blood samples screened positive for cannabinoids.John A. D’Asaro
Arizona Department of Public Safety, Central Regional Crime Laboratory, P.O. Box 6638, Phoenix, Arizona 85005

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