| |
Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 24,
Number 4, May/June 2000, pp.271-274
Blood
Concentrations of Amitriptyline and Its Metabolite in Rats after Acute Oral Administration
of Amitriptyline Amitriptyline
(AMT), a tricyclic antidepressant that is a dibenzocycloheptadine derivative,
is frequently used. However, the case reports of AMT-related fatalities are
increased, nowadays, due to the low levels of toxic and fatal concentration
in blood. So, this study was carried out to determine the concentrations of
AMT and its demethylated metabolite, nortriptyline (NTR), after acute single
oral administration of AMT in rats. Blood samples were collected five times
from the opthalmic venous plexus at 0, 1, 2, 4, and 8 h after acute single oral
administration of AMT in toxic doses of 10 (Group I) or 20 mg/kg (Group II),
and the concentrations of AMT and NTR and the mean ratios of AMT to NTR (AMT/NTR)
in the blood were periodically determined at designated times. The blood concentrations
of AMT and NTR were identified and quantitated by gas chromatography with thermionic
specific detection and gas chromatography–mass spectrometry after solid-phase
extraction with a Clean Screen DAU column. The peak blood concentrations of
AMT and NTR in Group I were 0.34 and 0.28 µg/mL, respectively, and those
of AMT and NTR in Group II were 0.59 and 0.43 µg/mL, respectively, and
were reached at 1 h after single oral administration.Seung-Kyung
Baeck*, Mie-Ae Lim, Seh-Youn Park, Ju-Seon Lee, Han-Sun Lee, and Ki-Ser Koo
Drug-Toxicology Division, Forensic Science Department, National Institute of Scientific
Investigation, 331-1, Sinwol 7-Dong, Yangcheon-Gu, Seoul, 158-097, Korea Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00 each.
Visa, MasterCard and AMEX accepted.
To
order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
To order multiple copies click here.
Please indicate JAT
volume and issue along with page numbers. |
|
Home | Subscribe
| Current Issue | Back Issues
| Search | Advertise | Other Publications
| |
