| |
Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 24,
Number 4, May/June 2000, pp. 233-237
Effects of
Pyridinium Chlorochromate Adulterant (Urine Luck) on Testing for Drugs of Abuse
and a Method for Quantitative Detection of Chromium (VI) in Urine* Pyridinium
chlorochromate (PCC) as an adulterant is popular for concealing drug-positive
results. When 11-nor-D9-THC-9-carboxylic acid (THC-acid)
in urine was treated with 2 mmol/L of PCC (Cr6+ 104 µg/mL), 58–100%
of the THC-acid was lost. The loss increased with decreasing pH and increasing
reaction time (0–3 days). Free codeine and free morphine remained unaffected
by PCC at pH within the physiological range of the urine (pH 5–7). At lower
pH, the loss of free morphine varied from 0 to 100%. Amphetamine, methamphetamine,
benzoylecgonine, and PCP remained unaffected by PCC when exposed to the oxidant
for three days in urine pH of 3–7. Chromium (VI) from PCC in a urine solution
was detected by a color reaction with 1,5-diphenylcarbazide (DPC). When the
reagent was added to the urine, an immediate red-violet color appeared. The
chromium-DPC complex showed a characteristic absorption peak at wavelength 544
nm with a shoulder at wavelength 575 nm. The ratio of absorption was used to
identify the chromium compound. The concentration of chromium (VI) was determined
by measuring absorption at wavelength 544 nm and was linear over 0.5–20
µg/mL. The limit of detection of the procedure was 0.37 µg/mL.Buddha
D. Paul, Karoline K. Martin, Joseph Maguilo, Jr., and Michael L. Smith
Division of Forensic Toxicology, Office of the Armed Forces Medical Examiner,
Armed Forces Institute of Pathology, Rockville, Maryland 20850 Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted. To order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
Please indicate JAT
volume and issue along with page numbers. |
|
Home | Subscribe
| Current Issue | Back Issues
| Search | Advertise | Other Publications
| |
