Published: Journal of Analytical Toxicology, Volume 24, Number 2, March, pp.93-96
Solid-Phase
Microextraction in the Determination of Methadone in Human Saliva by Gas Chromatography–Mass
Spectrometry
1Department
of Clinical Analysis and Toxicology, Faculty of Pharmacy, University of Amazonas,
Brazil and 2Department of Legal Medicine, Forensic Toxicology Service, Faculty
of Medicine, University of Santiago de Compostela, Spain
Solid-phase microextraction (SPME) with a 100-µm polydimethylsiloxane film fiber was applied to the determination of methadone and 2-ethylidine-3,3-diphenylpyrrolidine (EDDP) by GC–MS in human saliva and compared with liquid–liquid extraction. A shorter extraction time of 30 min with the fiber was obtained, speeding up the total analysis time. Linearity was found for SPME from 0.05 to 2.0 µg/mL (r = 0.9976 for methadone; r = 0.9988 for EDDP) with precision between 0.7 and 4.3% for saliva spiked with 0.2 and 1.5 µg/mL of methadone and EDDP. The limit of detection using SPME was 0.04 µg/mL for methadone and 0.008 µg/mL for EDDP. Analytical recoveries of SPME and liquid–liquid extraction ranged from 98.8 to 103.6%. The use of deuterated internal standard by both methods have yielded comparable results. Thus, the SPME method is highly accurate, precise, and useful for determination of methadone and EDDP in saliva.
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