The Determination of Æ9-Tetrahydrocannabinol and 11-Nor-9-Carboxy-Æ9-tetrahydrocannabinol in Whole Blood Using Solvent Extraction Combined with Polar Solid-Phase Extraction

Published: Journal of Analytical Toxicology, Volume 24, Number 2, March, pp.127-132

The Determination of D⁹-Tetrahydrocannabinol and 11-Nor-9-Carboxy-D⁹-tetrahydrocannabinol in Whole Blood Using Solvent Extraction Combined with Polar Solid-Phase Extraction
Peter D. Felgate and Andrew C. Dinan
Forensic Science, Department for Administrative and Information Services, Forensic Science Centre, 21 Divett Place, Adelaide, South Australia 5000, Australia

A method for the determination of D⁹-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-D⁹-tetrahydrocannabinol (THCCOOH) in whole blood using conventional solvent extraction followed by a cleanup using polar solid-phase cartridges is described. The method uses 0.5 mL of blood, and detection is by benchtop gas chromatography–mass selective detection using selected ion monitoring. The limit of detection is better than 1 ng/mL, and extraction efficiencies are greater than 80% for THC and 70% for THCCOOH. The method is robust and highly reproducible.

Reproduction of editorial content of this journal is prohibited without publisher’s permission.

This article is available in its entirety by fax for $4.00 per page.
Visa or MasterCard accepted.

To order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.

Please indicate JAT volume and issue along with page numbers.