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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 24,
Number 5, July/August, pp. 372-376
Here is where the title stuff goes
Rapid Analysis
of Halothane in Biological Samples using Headspace Solid-Phase Microextraction
and Gas Chromatography–Mass Spectrometry—A Case of a Double Homicide
Frank
Musshoff*, Heike Junker, and Burkhard Madea
Institute of Legal Medicine, Rheinische Friedrich-Wilhelms-University, Stiftsplatz
12, 53111 Bonn, Germany
A simple,
rapid, and sensitive method for the analysis of halothane in biological samples
was developed. The procedure describes the extraction of halothane from blood,
liver, kidney, brain, urine, bile, and stomach contents by headspace solid-phase
microextraction (HS-SPME) followed by capillary gas chromatography coupled
with mass spectrometry (GC–MS). The recovery in blood samples after addition
of ammonium sulfate and sulfuric acid was 72% compared to a sample prepared
in water (100%). Linearity was established over a concentration range of 0.1–100
mg/kg of spiked blood samples with an excellent coefficient of correlation
(0.996) and a limit of detection of 0.004 mg/kg. The time for analysis was
approximately 40 min per sample including the extraction step. The procedure
was used for quantitation of halothane in various samples in a case of a double
homicide. HS-SPME in combination with GC–MS was an effective method for
the determination and quantitation of halothane in biological material.
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