Published: Journal of Analytical Toxicology, ISSN 0146-4760, Volume 24, Number 5, July/August, pp. 359-364

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Enhanced Selectivity in the Determination of D⁹-Tetrahydrocannabinol and Two Major Metabolites in Serum Using Ion-Trap GC–MS–MS

J.-P. Weller1,*, M. Wolf1, and S. Szidat2
1Institute of Legal Medicine, Medical High-School Hannover, Germany and 2Center for Radiation Protection and Radioecology, University of Hannover, Germany

Cannabinoids were extracted from serum with C₁₈ SPE cartridges and analyzed as their trimethylsilyl (TMS) derivatives. A benchtop gas chromatography–tandem mass spectrometry (GC–MS–MS) system based on an ion trap with external ionization was used. Quantitation was done in relation to trideuterated internal standards in dual MS–MS mode. Confirmation of the identity for the three compounds of interest, D⁹-tetrahydrocannabinol (THC), 11-hydroxy-D⁹-tetrahydrocannabinol (11-OH-THC), and 11-nor-9-carboxy-D⁹-tetrahydrocannabinol (THCCOOH), was achieved by registering the daughter spectra in full scan mode. It was possible to identify the three compounds at concentrations down to 0.25 µg/L for THC, 0.5 µg/L for 11-OH-THC, and < 2.5 µg/L for THCCOOH by comparison with reference spectra. The limits of quantitation are better than 2 µg/L for THC, 5 µg/L for 11-OH-THC, and 8 µg/L for THCCOOH. The within-run and day-to-day precision for the three analytes were very similar and ranged from 4.2 to 10.4%.

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