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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 24,
Number 5, July/August, pp. 354-358
Here is where the title stuff goes
Discrimination
of Dimethylamphetamine and Methamphetamine Use: Simultaneous Determination
of Dimethylamphetamine-N-Oxide and Other Metabolites in Urine by High-Performance
Liquid Chromatography– Electrospray Ionization Mass Spectrometry
Munehiro
Katagi*, Michiaki Tatsuno, Akihiro Miki, Mayumi Nishikawa, and Hitoshi Tsuchihashi
Forensic Science Laboratory, Osaka Prefectural Police Headquarters, 3-18 Hommachi
1-Chome, Chuo-ward, Osaka 541-0053, Japan
A simple
and sensitive method by high-performance liquid chromatography–electrospray
ionization mass spectrometry (LC–ESI-MS) has been investigated for the
simultaneous determination of dimethylamphetamine (DMA), its specific yet
labile main metabolite dimethylamphetamine-N-oxide (DMAO), and other metabolites,
methamphetamine (MA) and amphetamine (AP), in urine. A combination of Bond
Elut SCX columns for the solid-phase extraction of urine and a semi-micro
SCX column for LC separations provided satisfactory results. The use of acetonitrile/5mM
ammonium acetate buffer adjusted to pH 4 (65:35, v/v) as the mobile phase
at a flow rate of 0.2 mL/min was found to be the most effective. The detection
limits were 5 ng/mL for DMAO, 10 ng/mL for DMA and MA, and 50 ng/mL for AP
in the SIM mode.
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