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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 24,
Number 5, July/August, pp. 328-332
Here is where the title stuff goes
Determination
of Methyl Mercury in Whole Blood by Ethylation–GC–CVAFS after Alkaline
Digestion–Solvent Extraction
L.
Liang1,*, C. Evens2, S. Lazoff1, J.S. Woods3, E. Cernichiari4, M. Horvat5,
M.D. Martin6, and T. DeRouen2
1Cebam Analytical, Inc., Seattle, Washington; 2Dental Public Sciences, University
of Washington, School of Dentistry, Seattle, Washington; 3Department of Environmental
Health, University of Washington, Seattle, Washington; 4Department of Environmental
Medicine, University of Rochester, New York; 5Department of Environmental
Sciences, J. Stefan Institute, Slovenia; and 6Department of Oral Medicine,
University of Washington, Seattle, Washington
A method
for the determination of methyl mercury in whole blood samples based on ethylation–gas
chromatography–cold vapor atomic fluorescence spectrometry after alkaline
digestion–solvent extraction is described. The extraction procedure and
conditions were optimized, and the matrix interference after extraction was
critically investigated. The storage stability of MeHg in blood samples and
a series of extracts was determined. The method detection limit was found
to be approximately 0.02 ng/g for a 0.5-g blood sample with relative standard
deviations of less than 10%. The accuracy and precision were evaluated by
summarizing the quality-control (QC) data generated over a one and one half
year period. Appropriate procedures for sample collection, transportation,
and storage were adapted to the method. Using this method accompanied by explicit
QC protocols and procedures, background levels of MeHg and total mercury in
blood for 150 8–10-year-old Portuguese children with nonoccupational
and nonamalgamal exposure were determined and reported with summarized QC
data.
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