Published: Journal of Analytical Toxicology, ISSN 0146-4760, Volume 24, Number 5, July/August, pp. 323-327

Here is where the title stuff goes

An Assay Evaluation of the Methylene Blue Method for the Detection of Anionic Surfactants in Urine

Joseph T. Jones and Francis M. Esposito
Laboratory Corporation of America Holdings, 1904 Alexander Drive, Research Triangle Park, North Carolina 27709

Adding detergent to urine intended for drug testing is one of many ways to adulterate the specimen. This modified methylene blue procedure allows the detection and quantitation of anionic surfactants in urine. One-hundred urine specimens that exhibited normal foaming when shaken gave anionic surfactant values lower than 36 µg/mL with a mean of 8.73 µg/mL. Most of the suspected adulterated specimens and spiked samples with only 100 µL of detergent in 60 mL of urine had values greater than 750 µg/mL. Based on the analysis of negative samples, a urine specimen with an anionic surfactant level of 100 µg/mL or greater could be considered adulterated and most likely will have levels greater than 800 µg/mL.

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