Published: Journal of Analytical Toxicology, Volume 24, Number 1, January/February, pp66-69
Use of Solid-Phase Microextraction (SPME) for the Determination
of Methadone and Its Main Metabolite, EDDP, in Plasma by Gas Chromatography–Mass
Spectrometry
A.M. Bermejo, R. Seara, A.C. dos Santos Lucas, M.J. Tabernero,
P. Fernández, and R. Marsili
Department of Legal
Medicine, Forensic Toxicology Service, Faculty of Medicine, Santiago de Compostela,
Spain;Department of Clinical Analysis and Toxicology, Faculty of Pharmacy, University
of Amazonas, Brazil; and Institute of Legal Medicine, Catholic University of
Rome, Italy
A simple, rapid method for the determination of methadone and its metabolite 2-ethylene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in plasma using solid-phase microextraction (SPME) and gas chromatography–mass spectrometry is proposed. A 100-µm polydimethylsiloxane film fiber was exposed by immersion for 30 min in a diluted plasma solution (1:4 with buffer pH 9) containing both compounds and an internal standard (proadifen). Calibration curves were linear over the concentration range 50–2000 ng/mL. The analysis time was 45 min per sample. The determination of methadone and EDDP was subject to no interference. The performance of SPME was compared with that of liquid–liquid extraction, obtaining lower limits of detection for EDDP. The method using the two extraction procedures was applied to 10 plasma samples from methadone-treated patients.
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