Published: Journal of Analytical Toxicology, Volume 24, Number 3, April, pp.170-179

The Quantitation of 2-Oxo-3-hydroxy Lysergic Acid Diethylamide (O-H-LSD) in Human Urine Specimens, a Metabolite of LSD: Comparative Analysis Using Liquid Chromatography–Selected Ion Monitoring Mass Spectrometry and Liquid Chromatography–Ion Trap Mass Spectrometry
Gregory K. Poch, Kevin L. Klette, and Cynthia Anderson
Navy Drug Screening Laboratory, 34425 Farenholt Avenue, Suite 40, San Diego, California 92134-7040

This paper compares the potential forensic application of two sensitive and rapid procedures (liquid chromatography–mass spectrometry and liquid chromatography–ion trap mass spectrometry) for the detection and quantitation of 2-oxo-3-hydroxy lysergic acid diethylamide (O-H-LSD) a major LSD metabolite. O-H-LSD calibration curves for both procedures were linear over the concentration range 0–8000 pg/mL with correlation coefficients (r²) greater than 0.99. The observed limit of detection (LOD) and limit of quantitation (LOQ) for O-H-LSD in both procedures was 400 pg/mL. Sixty-eight human urine specimens that had previously been found to contain LSD by gas chromatography–mass spectrometry were reanalyzed by both procedures for LSD and O-H-LSD. These specimens contained a mean concentration of O-H-LSD approximately 16 times higher than the LSD concentration. Because both LC methods produce similar results, either procedure can be readily adapted to O-H-LSD analysis for use in high-volume drug-testing laboratories. In addition, the possibility of significantly increasing the LSD detection time window by targeting this major LSD metabolite for analysis may influence other drug-free workplace programs to test for LSD.

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