Published: Journal of Analytical Toxicology, Volume 23, Number 5, September 1999, pp.301-305 .
Analysis of Gamma-Hydroxybutyrate (GHB) in Urine by Gas Chromatography–Mass Spectrometry
Rachel R. McCusker, Helen Paget-Wilkes, Chris W. Chronister, and Bruce A. Goldberger*, and Mahmoud A. ElSohly
A simple method for the direct analysis of gamma-hydroxybutyrate (GHB) from human urine is described. The method uses solid-phase extraction, liquid–liquid extraction, and silyl-derivatization, then gas chromatographic–mass spectrometric analysis using GHB-d6 as the internal standard. The method was linear from 5 to 500 mg/L, and coefficients of variation were less than 10%. Twenty-six urine specimens previously analyzed by an existing method were analyzed and yielded GHB concentrations ranging from 0 to 6100 mg/L; the results correlated between the two methods. Compared with existing methods, the method described here is superior because it is specific to GHB and can discriminate between GHB and gamma-butyrolactone.Reproduction of editorial content of this journal is prohibited without publisher’s permission.
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