Published: Journal of Analytical Toxicology, Volume
23,
Number 6,
October 1999,
pp.518-523
Simultaneous Determination of Amphetamine, Methamphetamine, Methylenedioxyamphetamine
(MDA), Methylenedioxymethamphetamine (MDMA), and Methylenedioxyethylamphetamine
(MDEA) Enantiomers by GC–MS*
Donna Hensley and John T. Cody
A method is described for the simultaneous determination of the ratio of l- and
d-enantiomers of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA),
3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine
(MDEA) in urine. The assay uses liquid–liquid extraction followed by derivatization
with trifluoroacetyl-l-prolyl chloride (l-TPC) and analysis by gas chromatography–mass
spectrometry. The assay was developed using prepared samples containing varying
concentrations of each of the analytes over a range of percentages of each enantiomer.
Results showed the method to provide accurate and reliable results in samples
containing ³ 10 ng/mL amphetamine and methamphetamine and ³ 25 ng/mL MDA, MDMA,
and MDEA. The assay was used to analyze urine samples from subjects of a controlled
MDMA study. Results for each of the eight subjects showed a greater percentage
of the l-enantiomer of MDMA initially, and the percentage increased with time
postdose. Analysis of the metabolite MDA revealed that the proportion of d-enantiomer
was initially greater than the l-enantiomer followed by a gradual increase in
the proportion of l-enantiomer until it exceeded the amount of the d-enantiomer.
In all cases, the l-MDA exceeded the d-MDA within the first 36 h postdose.
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