Published: Journal of Analytical Toxicology, Volume 23, Number 7, Nov/Dec 1999, pp. 632-635
Rapid Extraction of Clenbuterol from Human and Calf Urine
Using Empore™ C8 Extraction Disks
In the present paper, disk extraction was evaluated for the rapid isolation of clenbuterol from human and calf urine, followed by high-performance liquid chromatography analysis with UV detection. A method was developed for the extraction with standard density C8 disks. The disks could be washed with 25% methanol in 0.01M sodium hydroxide without significant losses of clenbuterol. The recovery of clenbuterol was about 85%, and the extracts were clean. The detection limit was about 10 ng/mL. The main advantages of these disks were the saving of time and the reduced amounts of organic solvents needed.
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