Published: Journal of Analytical Toxicology, Volume 23, Number 7, Nov/Dec 1999, pp. 598-602
On-Line Immunoaffinity Extraction and HPLC Analysis of
Flunitrazepam and Its Main Metabolites in Serum
A sensitive, simple, and rapid method for the determination of flunitrazepam and its major metabolites (7-aminoflunitrazepam, 7-acetamidoflunitrazepam, and norflunitrazepam) in serum and plasma is presented. The on-line procedure uses an immobilized, highly reusable antibody against benzodiazepines for selective extraction from serum followed by analysis by high-performance liquid chromatography with ultraviolet detection. This reliable method provides a limit of detection of 1 ng/mL serum, and results are obtained in less than 40 min.
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