Published: Journal of Analytical Toxicology, Volume
23,
Number 4, July/August
1999, pp.257-261 .
Determination of Monomethylarsonic Acid and Dimethylarsinic
Acid in Urine by Liquid Chromatography–Tandem Mass Spectrometry
Anne Le Bouil, Annie Cailleux, Alain Turcant, and Pierre Allain
A method for the simultaneous measurement of monomethylarsonic acid (MMA) and
dimethylarsinic acid (DMA) in human urine using liquid chromatography–tandem
mass spectrometry with electrospray ionization (LC–ES-MS–MS) was developed.
The multiple reaction monitoring mode (MRM) was used for quantitation. The protonated
molecule ions (m/z 141.0 for MMA and m/z 139.0 for DMA) were selected as precursor
ions, and the same fragment ion AsO+ (m/z 91.1) was monitored as the product ion.
A two-step liquid–liquid extraction of MMA and DMA from urine provided recoveries
of 92–100%. The coefficients of variation were lower than 7% for the within-day
precision and lower than 11% for the between-day precision. The limit of quantitation
was 25 µg/L as As for the two analytes. The assay was linear over the range
of 25–800 µg/L.
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