Published: Journal of Analytical Toxicology, Volume 23, Number 1, January/February 1999, pp. 1-6.
Analysis of Gabapentin in Serum and Plasma by Solid-Phase
Extraction and Gas Chromatography–Mass Spectrometry for Therapeutic Drug
Monitoring
M.M. Kushnir, J. Crossett, P.I. Brown, and F.M. Urry
A simple method for the determination of gabapentin (Neurontin®) is described.
The method uses solid-phase extraction by disk column and derivatization followed
by gas chromatographic–mass spectrometric analysis. The single-step derivatization
with MTBSTFA produces a t-BDMS derivative of both the carboxylic and amine moieties
of the molecule. Each step of the procedure was optimized to assure reliable
performance of the method. The assay limit of detection was 0.1 µg/mL
with a linear range from 1.0 to 35 µg/mL. Within-run (n = 3) and between-run
(n = 40) coefficients of variation were less than 8.2 and 15.9%, respectively.
The method has proven reliable in routine production for more than a year, producing
clean chromatography with unique ion fragments, consistent ion mass ratios,
and no interferences. Statistical analysis of the gabapentin concentrations
measured in 1020 random specimens over a 2-month period showed a mean concentration
of 6.07 µg/mL with a standard deviation of 5.28.
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