Published: Journal of Analytical Toxicology, Volume 22, Number 7, November/December 1998, pp. 601-604
Evaluation of the Diagnostic Performance of the Boehringer
Mannheim CEDIA® LSD Assay
Alain G. Verstraete and Sophia Steyaert
The precision and the diagnostic performance of the Boehringer Mannheim CEDIA
DAU LSD assay was evaluated. The assay was performed in the semi-quantitative
mode on a Hitachi 917 analyzer. Within-run coefficients of variation (CVs) of
the semiquantitative values for 0.25 and 1.0 ng/mL were 11.2 and 6.2%, respectively.
Day-to-day CVs for the same concentrations were 12.6 and 8.6%. We analyzed 318
urine samples by CEDIA, DPC® Coat-A-Count® RIA and Behring EMIT®II.
Confirmation was performed by GC–MS, after extraction on Bond Elut Certify®
columns. Two hundred sixty-three samples were negative by all methods. Twenty-five
samples were positive by all immunoassays, 19 of which were confirmed by gas
chromatography–mass spectrometry (GC–MS). One sample was falsely negative
by CEDIA. Three samples were positive by EMIT and CEDIA, but negative by RIA
and GC–MS. Twenty-six samples were positive by EMIT alone, but they were
not confirmed by GC–MS.
The LSD CEDIA assay seems to be less specific than DPC RIA but more specific
than the EMIT LSD assay.
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