Published: Journal of Analytical Toxicology, Volume 22, Number 3, May/June 1998, pp. 197–202.
Determination of Flunitrazepam and its
Main Metabolites in Serum and Urine by HPLC after Mixed-Mode
Solid-Phase Extraction
I. Deinl, G. Mahr, and L. von
Meyer
A rapid and sensitive method is presented for the simultaneous determination of flunitrazepam, norflunitrazepam, 7-aminoflunitrazepam, and 7-acetamidoflunitrazepam in serum, plasma, and urine. The compounds were extracted by a mixed-mode solid-phase procedure following analysis by high-performance liquid chromatography and ultraviolet detection and using methylclonazepam as the internal standard. The method revealed high recoveries and showed good precision and linearity for all compounds. The limit of detection was at least 1 ng/mL serum (plasma) for all compounds.
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