Published: Journal of Analytical Toxicology, Volume 22, Number 2, March/April 1998, pp. 105–111.
A Barbiturate Screening Assay for the
Abbott AxSYM Analyzer
M. Adamczyk, J. Douglas, J.
Grote, and C.A. Harrington
A fluorescence polarization immunoassay for barbiturates on the Abbott AxSYM analyzer is described. The assay displayed dilution linearity up to 1200 ng/mL; coefficients of variation varied from 5.96 to 8.61%; recovery varied from 94.9 to 105.3%; and sensitivity was less than 40 ng/mL. Good correlation between the standard six- and factory two-point calibration methods was observed. The immunoassay demonstrated good cross-reactivity to several commonly prescribed barbiturates; low cross-reactivity with structurally similar compounds; low interference from endogeneous substances, dyes, preservatives, and several commonly available adulterants; and good correlation with the TDx Barbiturate Urine assay.
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