Published: Journal of Analytical Toxicology, Volume 21, Number 6, October 1997, pp. 433–437.
Evaluation of Reduced
Solvent Volume Solid-Phase Extraction Columns with Analysis by
Gas Chromatography–Mass Spectrometry for Determination of
11-nor-9-Carboxy-D9-THC in Urine
Lisa
O’Dell, Kathy Rymut, Glynn Chaney, Tony Darpino, and Michael
Telepchak
A sensitive and reliable method was developed
for the identification and quantitation of 11-nor-9-carboxy-D9-tetrahydrocannabinol
(THCCOOH) in urine using a microbed solid-phase extraction (SPE)
column. Extensive method validation is presented that evaluates
the recovery, linearity, precision, limit of quantitation, limit
of detection, and capacity of THCCOOH in urine extracts using
CLEAN SCREEN®,1 reduced solvent volume
(RSV) SPE columns followed by gas chromatographic–mass
spectrometric analysis. The mean recovery of THCCOOH at
concentrations of
2, 3, 5, 15, 50, and 150 ng/mL was found to be 91% with
coefficients of variation of 7.3% or less. Linearity of the
method ranged from 1.95 to 1000 ng/mL with sensitivity at 1 ng/mL
for THCCOOH. The sorbent was found to retain at least 1000 ng/mL
of THCCOOH with no analyte breakthrough using the described
method. Reduction in both processing times and total solvent
volume is shown. The RSV SPE columns showed excellent efficiency
and performance while demonstrating recoveries, cleanliness, and
dynamic ranges comparable with standard SPE products.
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