Published: Journal of Analytical Toxicology, Volume 21, Number 7, November/December 1997, pp. 548–553.
Reversed-Phase HPLC Determination of
Eight Anticoagulant Rodenticides in Animal Liver
V. Fauconnet, H. Pouliquen,
and L. Pinault
A reversed-phase high-performance liquid chromatographic method was developed for the analysis of eight anticoagulant rodenticides in animal liver. Coumarinic anticoagulant rodenticides (brodifacoum, bromadiolone, coumachlor, coumatetralyl, difenacoum, and warfarin) were detected by using a gradient elution and a fluorimetric detection. Indanedione anticoagulant rodenticides (chlorophacinone and diphacinone) were detected by using an isocratic elution and an UV detection. Anticoagulants were extracted from liver with mixtures of acetone/diethylether and acetone/chloroform. Extracts were applied to solid-phase extraction cartridges. Linearity was checked over the concentration range 0.1–0.6 µg/g. Relative standard deviations of within-run and between-run variability were all between 5.7 and 10.3%. Recoveries from spiked liver samples were between 51.7 (difenacoum) and 78.2% (warfarin). Limits of detection were between 0.01 (difenacoum and warfarin) and 0.11 µg/g (chlorophacinone).
Reproduction of editorial content of this journal is prohibited without publisher’s permission.
| This article is available in its entirety by fax for $40.00 each. Visa, MasterCard and AMEX accepted. To order electronically click
here Please indicate JAT volume and issue along with page numbers. |
| Subscribe | Current Issue | Back Issues | Search | Advertise | Other Publications |