Published: Journal of Analytical Toxicology, Volume 21, Number 3, May/June 1997, pp. 208–212.
Detection of
2-Amino-5-Chloropyridine in Urine as a Parameter of Zopiclone
(Imovane®) Intake
using HPLC with Diode Array Detection
E. Mannaert,
J. Tytgat, and P. Daenens
A qualitative screening technique was developed for the detection of 2-amino-5-chloropyridine, a newly identified decomposition product of zopiclone and metabolites after alkaline hydrolysis of urine samples. The method was elaborated using a standard operation procedure (Merck Tox Screening System), combining a solid-phase extraction with reversed-phase high-performance liquid chromatography and diode array detection. The limit of detection, expressed as zopiclone concentrations in spiked urine, was found to be 0.5 µg/mL. Field urine samples from a volunteer were positive for 2-amino-5-chloropyridine until 16 h after ingestion of one therapeutic dose of Imovane (7.5 mg zopiclone).
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