Published: Journal of Analytical Toxicology, Volume 21, Number 4, July/August 1997, pp. 278–282.
Solid-Phase Extraction
in Amphetamine and Methamphetamine Analysis of Urine
M.-R. Lee, S.-C. Yu, C.-L.
Lin, Y.-C. Yeh, Y.-L. Chen, and S.-H. Hu
A systematic evaluation that compares the recoveries of methamphetamine and amphetamine from spiked urine using C8, C18, strong cation exchanger (SCX), and C8–SCX mixed procedures (solid-phase extraction) is reported. Optimized experimental conditions including pH of the sample, solvent composition, and urine concentration for different solid-phase extraction sorbents were studied. The largest recoveries—greater than 86% for methamphetamine and greater than 88% for amphetamine—were achieved with a mixed adsorbent (C8–SCX) and mixed elution solvent CH2Cl2–i-propanol–NH4OH (78:20:2) at pH 6. The concentration of the urine matrix did not affect the extraction efficiency. The detection limits of methamphetamine and amphetamine in urine were obtained at 0.03 and 0.07 µg/mL, respectively, using gas chromatographic–mass spectrometric analysis operated in the electron impact mode.
Reproduction of editorial content of this journal is prohibited without publisher’s permission.
| This article is available in its entirety by fax for $4.00 per page. Visa or MasterCard accepted. To order electronically click here Please indicate JAT volume and issue along with page numbers. |
| Subscribe | Current Issue | Back Issues | Search | Advertise | Other Publications |