Published: Journal of Analytical Toxicology, Volume 20, Number 5, September 1996, pp. 318-323.
Determination of Ester-Type Local Anesthetic Drugs (Procaine,
tetracaine, and T-caine) in Human Serum by Wide-Bore Capillary Gas Chromatography
with Nitrogen-Phosphorus Detection
Masaru Terada, Mohammed N. Islam, Zaw Tun, Katsuya Honda, Choei Wakasugi,
Tatsuo Shinozuka, Jun-ichi Yanagida, Toshinori Yamamoto and Yukio Kuroiwa
A sensitive method for simultaneous determination of ester-type local anesthetic drugs (procaine, tetracaine, and T-caine) has been developed using wide-bore capillary gas chromatography with nitrogen-phosphorus detection (GC–NPD). The extraction procedure, the experimental conditions for heptafluorobutyryl (HFB) derivative formation, and the percentage of the ester-type local anesthetic drugs from the human serum are described. The HFB derivatives of ester-type local anesthetic drugs showed sensitivity of approximately 2–3-fold higher than that without derivatization. The detection limits of HFB derivatives of the ester-type local anesthetic drugs were approximately 60–70 pg on column. Recoveries from the human serum were 85–94%. This method could be used to determine concentrations as low as 24–28 ng/mL of the ester-type local anesthetic drugs.
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