Published: Journal of Analytical Toxicology, Volume 20, Number 5, September 1996, pp. 305-308.
Comparison of HPLC and GC–MS for Measurement of Cocaine
and Metabolites in Human Urine
Diane L. Phillips, Ian R. Tebbett, Roger and L. Bertholf
High-performance liquid chromatography (HPLC) was compared with gas chromatography–mass spectrometry (GC–MS) for quantitation of cocaine, benzoylecgonine, norcocaine, and cocaethylene in urine. Calibration standards were prepared in human urine, and bupivacaine was added as the internal standard for quantitation. After solid-phase extraction, the reconstituted samples were divided into aliquots for analysis by HPLC and GC–MS. The analytical performance of the two methods were compared with regard to sensitivity, precision, and dynamic range. Results of GC–MS and HPLC analyses of nine urine specimens previously confirmed as positive for benzoylecgonine were compared. Analytical results by HPLC were comparable to GC–MS. Therefore, for many laboratories, HPLC is a useful alternative to GC–MS for measuring cocaine and metabolites in urine.
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