Investigation of the Effects of Solution Composition and Container Material ...

Published: Journal of Analytical Toxicology, Volume 20, Number 5, September 1996, pp. 291-300.

Investigation of the Effects of Solution Composition and Container Material Type on the Loss of 11-nor-D9-THC-9-Carboxylic Acid
Kenneth D.W. Roth, Neal A. Siegel, Robert W. Johnson, Jr., László Litauszki, Lawrence Salvati, Jr., Charles A. Harrington, and Larry K. Wray

The loss of 11-nor-D9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) from solution was studied using fluorescence polarization immunoassay (FPIA) technology and x-ray photoelectron spectroscopy (XPS). Several materials (glass, silylated glass, high density polyethylene, polypropylene, polystyrene, polymethylmethacrylate, Teflon®, and Kynar®) were studied along with three solvents (water, urine, and Abbott® cannabinoids diluent). THC-COOH losses ranging from 0 to 9.7 ng/cm2 and concentration reductions to 46% of starting values were measured. XPS indicated the presence of fluorine-labeled THC-COOH at materials surfaces. A half-life of 10 min was calculated for THC-COOH loss from urine stored in high density polyethylene at room temperature. Sample handling losses during pipetting were determined and ranged from 1.1 to 7.9 ng per aliquot. The effects of sample volume and sample handling on the THC-COOH concentrations of controls were also investigated.

Reproduction of editorial content of this journal is prohibited without publisher’s permission.

This article is available in its entirety by fax for $40.00 each.
Visa, MasterCard and AMEX accepted.

To order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.

Please indicate JAT volume and issue along with page numbers.