Determination of Phenmetrazine in Urine by Gas ChromatographyMass Spectrometry

Published: Journal of Analytical Toxicology, Volume 20, Number 5, September 1996, pp. 277-280.

Determination of Phenmetrazine in Urine by Gas Chromatography–Mass Spectrometry
Robert Kronstrand, Marko Hatanpää, and John A. Jonsson

A sensitive and simple gas chromatographic–mass spectrometric method is described for the determination of the central nervous system (CNS) stimulant phenmetrazine in urine. The extraction and derivatization were combined into a single step with isooctane and methyl chloroformate. The limit of quantitation was 0.05 µg/mL urine, and the method was linear up to 100 µg/mL. The coefficients of variation (CV) for within-day runs were 1.2% and 2.4% (n = 5) for two controls containing 1.0 µg/mL and 50 µg/mL, respectively. During a six-month period, the same controls showed CVs of 9.1% and 8.7%, respectively (n = 40), indicating a somewhat lower between-run precision. Phenmetrazine was present in 83 out of 3000 urine samples that were screened for CNS stimulants during this period, and the concentrations ranged from 0.5–370 µg/mL.

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