Published: Journal of Analytical Toxicology, Volume 20, Number 6, October 1996, pp. 506-508.
One-Step Esterification of Benzoylecgonine with Dimethylformamide–Dipropylacetal
or Dimethylformamide–Disopropylacetal in the Presence of Pyridine
Buddha D. Paul, Catherine Dreka, Jacqueline L. Summers, and Michael L. Smith
A simple procedure was developed to derivatize benzoylecgonine extracted from urine for subsequent confirmation by gas chromatography–mass spectrometry. The compound was esterified with dimethylformamide–dipropylacetal (DMF–DPA) or dimethylformamide–diisopropylacetal (DMF–DIPA) to the corresponding propyl and isopropyl esters. The optimum reaction condition was found to be heating the reaction mixture in the presence of pyridine at 100°C for 30 min. The procedure is a one-step esterification followed by evaporation of excess reagents. When benzoylecgonine was extracted from urine using a solid-phase extraction technique and derivatized with this procedure, the compound was detected at a level as low as 10 ng/mL. Quantitation was linear over the concentration range 10–8000 ng/mL.
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