Published: Journal of Analytical Toxicology, Volume 20, Number 3, May/June 1996, pp. 151-154.
Gas Chromatographic Determination of Parathion and Paraoxon
in Fish Plasma and Tissues
Richat Abbas and William L. Hayton
A sensitive capillary gas chromatographic (GC) method for the simultaneous determination of the organophosphate insecticide, parathion, and its active metabolite, paraoxon, in biological samples was developed. This method involved a simple liquid–liquid extraction of parathion and paraoxon from water, plasma, or tissues and capillary GC determination using electron-capture detection and splitless injection; malathion was used as the internal standard. A gradient oven temperature program was used; the injection port and detector temperatures were 200 and 300°C, respectively. These techniques allowed quantitative determination of parathion and paraoxon at 9–210-ng/mL concentrations; recoveries ranged from 79.4 to 110.3% for tissues and from 91.9 to 100.0% for plasma and water. The within-day and between-day coefficients of variation were less than 8.0%. The method was used to characterize the pharmacokinetics of parathion and paraoxon and the tissue distribution of paraoxon in rainbow trout.
Reproduction of editorial content of this journal is prohibited without publisher’s permission.
| This article is available in its entirety by fax for $40.00 each. Visa, MasterCard and AMEX accepted. To order electronically click
here Please indicate JAT volume and issue along with page numbers. |
| Home | Subscribe | Current Issue | Back Issues | Search | Advertise | Other Publications | Site Map |