Published: Journal of Analytical Toxicology, Volume 20, Number 2, March/April 1996, pp. 93-100.
A Screening Procedure for the Determination
of 13 Oral Anticoagulants and Rodenticides
H. Lotfi, M.F. Dreyfuss, P. Marquet, J. Debord, L. Merle, and G. Lachâtre
A technique for the simultaneous identification and quantitation
of 13 hydroxycoumarin and indandione anticoagulant drugs and rodenticides from
human serum by reversed-phase liquid chromatography with diode-array detection
has been developed. High-performance liquid chromatography was performed using
gradient elution with an acetonitrile and phosphate buffer on a Nucleosil ODS
column. Ultraviolet spectra from 200 to 400 nm were recorded on-line during
the analysis and compared with spectra stored in a library. For the spiked 2
mL of serum, acidic and alkaline liquid–liquid double extraction with diethylether–ether
acetate (50:50, v/v) was conducted, and recoveries greater than 60% for most
compounds were found. The detection limit was approximately 25 or 50 ng/mL for
all components except for difethialone and fluindione, for which it was approximately
100 ng/mL. The standard calibration curves were linear from the detection limit
to 5000 ng/mL. The within-run precision coefficient of variation (CV) was less
than 10%, and the between-run precision CV was less than 20%.
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