Published: Journal of Analytical Toxicology, Volume 20, Number 4, July/August 1996, pp. 262-265.
Solid-Phase Extraction and On-Disc Derivatization of the
Major Benzodiazepines in Urine using Enzyme Hydrolysis and Toxi-Lab VC MP3 Column
James W. King, and Leslie J. King
Because of the increase in use of the newer benzodiazepines, we explored the opportunity to develop a gas chromatographic–mass spectrometric (GC–MS) method that encompasses most of the widely prescribed benzodiazepines in use today. The benzodiazepines included in our study are nordiazepam, oxazepam, temazepam, lorazepam, a-hydroxyalprazolam, a-hydroxytriazolam, desalkylflurazepam, and 2-hydroxyethylflurazepam. Using 1.0 mL of urine as the matrix, we added the enzyme Glusulase and incubated the specimens for 2 h to obtain the free drugs. The hydrolyzed samples were then loaded onto a Toxi-Lab Spec VC MP3 column containing a 15-mg disc. On-disc derivatization was accomplished by adding N-methyl-N-(t-butyldimethylsilyl) trifluroacetamide (MTBSTFA) with 1% TBDMSCI to the disc. The derivatives were then placed in a GC vial and analyzed by GC–MS in the selected ion monitoring mode. These results were then compared to confirmed positives by the traditional acid hydrolysis GC–MS method.
Reproduction of editorial content of this journal is prohibited without publisher’s permission.
| This article is available in its entirety by fax for $40.00 each. Visa, MasterCard and AMEX accepted. To order electronically click
here Please indicate JAT volume and issue along with page numbers. |
| Home | Subscribe | Current Issue | Back Issues | Search | Advertise | Other Publications |